Spectranet customer portalSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. . This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Danaher Inc spectramax i3x. Price from $9. Plaque Reduction Neutralization Test (PRNT) SARS-CoV-2 neutralization was assessed as previously described . +44-118-944-8000. . The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 5577 Web: Email: info@moldev. StakMax Handling Stacker Base Unit. Biochrom New EX Read 400 Microplate Reader. Find regional support. D 1 1. The dual auto injector module. SeeSpectraMax MiniMaxImagingCytometeronpage85. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Actual shake speed is based on the microplate format. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. *On the SpectraMax i3x reader only. Supplier: Molecular Devices. Order Now. 1. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Citation 2002). These robust readers have been placed in labs from Antarctica to the. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. Structured Review. Version 7. We would like to show you a description here but the site won’t allow us. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. FTIR Spectrometer: Shimadzu IRSpirit (2018)The plate was read on the SpectraMax i3x Multi-Mode Microplate Reader. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Modularity does provide some measure of forward vision into upcoming or future experimental needs. . The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Product Details. 因为是导师第一任学生，没有师兄师姐，加上本人悟性也差，小小pcr花了半年时间才终于有点样子。因为经历过，所以懂得，故而分享之，希望能给到屏幕前的你一点点帮助! 如有失误，欢迎指正~最后，不要忘了一键三联哟，谢谢大家！, 视频播放量 6293、弹幕量 2、点赞数 122、投硬币枚数 55、收藏. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Figure 3. Videos & Webinars. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Assay setup for a 384-well low-volume plate. Bioz Stars score: 98/100, based on 1 PubMed citations. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 0. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Find regional support. Also refer to this Multimode Reader Comparison Chart. pathways and protein activation and expression in one system. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 313 reference gain check fail. Restart the system. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. 58% for the detection module and 2. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. . The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. MC38 in vivo tumor model in hPD-1. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. 001 0. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The M5 can read volumes down to 2uL with the uMax Low. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. See SpectraMax® Microplate Reader User Manual Download Page. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Expanded dynamic range Engineered for performance with spectral fusion illumination for increased sensitivity across the entire excitation range. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Quantiferon-TB Gold in Tube Kit. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. Examples: “Press carefully to engage,” “ Do not press down. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. SpectraMax® i3x. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. . Methods. 더 많은. Figure 1. (3) Molecular Devices, SpectraMax i3x, Microplate Readers (2) 2022 (1) 2020 Invetech Korus KRS-100 Elutriation & Washing Systems (3) 2021 ThermoFisher Scientific, invitrogen, CytKick Max AutoSampler w/ Attune NxT: Acea NovoCyte Flow Cytometer: Appliedbiosystems, QuantStudio 7 Flex, Real-Time PCR SystemThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Prime function 20 amol. The treatments were. With optimized reagents, option to operate the readers. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringFluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). Read modes. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. 2in. 012 fmol 0. Shaking and incubations were performed using an on-deck orbital shaker. SeeThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Prepare your iD series instrument for shipment. Each heating element is independently. SpectraMax Microplate Readers. SpectraMax iD3 and iD5 Register or Sign In. EUROPE. Description 1. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. Image analysis provides accurate data. Italic and boldface type are used for emphasis. Catalog excerpts. 306 plate air cal fail,low light. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Molecular Devicesの全商品を見る. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. SpectraMax M5. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. 蛍光 発光 ELISA. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Molecular Devices ScanLater Western Blot Detection Platform. Sufficient for 1000 reactions. . 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. See Unpacking the SpectraMax Paradigm Instrument on page 1. pH-Xtra Glycolysis Assay - Agilent. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Supplier: Molecular Devices. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Cet appareil ISM est confomre à la norme NMB-001 du Canada. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. TR-FRET ratios were calculated by dividing the RFU value for the 665-nm reading by the RFU value for the 616-nm reading, and the resulting numbers were multiplied by 1000. SpectraMax Quant dsDNA Assay Kits. 3) At the bottom of the strip, click on "Show. SpectraMaxi3Multi. . . SpectraMax® Microplate Readers and SoftMax® Pro Software. Use the 3. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. o For example: Ex: 485/Em 525/Cutoff 515 4. Summary of Contents for Molecular Devices SpectraMax M2. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 2 nM was calculated using SoftMax Pro Software. . The SpectraMax M4 reader had identical performance specifications to the SpectraMaxLuminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. All the readers were operated using SoftMax® Pro Software, which also has built-in data analysis and curve-fitting for automatic calculation of EC 50. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. The SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. Recommended settings for SpectraMax iD5 and i3x readers. The plate was covered and incubated for one hour at room temperature. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. 5 decades, with sensitive detection of cells. Description 1. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 4. Microplate readers, multi-mode, SpectraMax® i3x. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. 304 signal level saturation. Replicate values were averaged and imported into Design-Expert 8. Microplate Spectrophotometers. Winnersh Triangle. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. 吸収度マイクロプレートリーダー SpectraMax® Mini. Reader spectramax microplate id3 molecular devices multi mode boundaries functionality instrumentSpectramax i3x multi-mode microplate reader with minimax imaging Molecular devices spectramax i3x 使用される 販売のために 価格. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. StackMax Microplate Handling System. Then, cells were washed with PBS three times and 100 μL of Nano-Glo HiBiT Lytic Reagent was added to. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. iD5. . Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Both Microplate Optimization and Read Height Adjustment were. 99 to $1999. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. 0´ 104 1. Multimode Readers Comparison Chart. 3. SpectraMax i3xは、吸光・蛍光・発光スペクトル測定のためのマルチモードマイクロプレートリーダーです。. Background subtraction was not necessary. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Description 1. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. Explore a wealth of applications with the future-ready SpectraMax i3x. Example: [Setup]. 3: The cuvette chamber. Genscript cPass SARS-CoV-2 Neutralization Antibody Detection. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. SoftMax Pro. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringLuciferase activity was measured using a SpectraMax i3x microplate reader (Molecular Devices). . The TRF intensities measured are proportional to the level of extracellular acidification in each sample. 1 and above:. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 2 Note Section Patch:1) Select the correct plate and the wells that you want to measure. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 100 fmol 384-well (75 µL) 0. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. 00800 665 32860. Microplate Reader Shelf. Bioz Stars score: 98/100, based on 1 PubMed citations. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Explore a wealth of applications with the future-ready SpectraMax i3x. TaqMan SNP. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. As an aspirant Bioscience research with expertise in cell biology, immunology and bio instrumentation techniques, I bring a wealth of knowledge and expertise to the table. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. Published Dec 16, 2020 | Updated Oct 10, 2023. Background noise is reduced by the reader’s cooled PMT, enabling increased sensitivity and dynamic range. Prepare your i3/i3x instrument for shipment. Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. Supported Windows Operating System: See release notes. 1 Microplate Data Acquisition & Analysis Software for Windows. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. . . Results were plotted as ratio of green/red vs. 3: The cuvette chamber. . The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. Download PDF. Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. The dynamic range of the assay is about 3. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 313 reference gain check fail. compound concentration, and an IC 50 value of 21. . Figure 1. Time-. i3. No accessories are needed and SoftMax® Pro. The SpectraMax i3x reader provides theSoftMaxProSoftwareFormulaReferenceGuide 8 5049093A SummaryFormulas Summariesconsistoffourparts: ASummaryname(whichisusedtorefertotheSummaryinotherformulas). Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Italic and boldface type are used for emphasis. 99. . Analysis. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. Download PDF. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Pancreatic lipase activity was determined by measuring the absorbance of the test solution at 405 nm with a micro plate reader (MD SpectraMax i3x). *On the SpectraMax i3x reader only. HeLa cells were plated at 15,000 cells per well in 100 µL. Failed to load PDF file. Introduction. It allows you to explore cellular pathways and protein activation and expression in one system. Fractional occupancy Corrected BRET ratio NanoBRET. SpectraMax i3x, onboard luminescence 121. . SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Not compatible with Home Edition. Wait 20 seconds. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. 4. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Open the catalog to page 2. . For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. 99. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析We would like to show you a description here but the site won’t allow us. Description 1. . ; The top 3 importers of Spectramax are India with 365. The plate was shaken and incubated in the dark for 10 minutes. 0´ 104 8. . Data Analysis. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. gen) on a Spectramax i3X luminometer. Other technologies such as imaging. com. 22 μ m fi ltration. 1. D 1 1. i3x Specifications. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. 1. Curcumin binding assay. . ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. The calculated LOQ values were 0. 303 unable to cal dark current. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. china@moldev. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. 0 mm hex key. SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Serial number 33 900 01-3387. Bulk Kit. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. 312 gain calibration failed. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. Ease of use. Find our technical support information along with contact details. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. SpectraMax i3x Multi-Mode Detection Platform. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. to View Figures. . 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Spectranet customer portalThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. 030 fmol. Request a Quote for This Setup. Detection cartridge modularity allows for easy addition of new read modes within minutes without. MitoXpress-Xtra Oxygen Consumption Assay - Agilent. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 12911151) 0. 303 unable to cal dark current. Engineered for high performance, the auto dual injector cartridge includes fail safe features. ValitaTiter Plus settingsContact our support team with your questions regarding Molecular Devices products and applications. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Table 1. . Monday to Friday 7am - 5pm Pacific Time. D 1 1. Figure 4. Filterless readers provide consistent results with steady temperature regulation.